The “Aseptic” experiment aboard the International Space Station (ISS) aims to experimentally evaluate the effectiveness of aseptic conditions during biotech experiments in a crewed spaceflight environment. Conducted by Roscosmos astronaut Ivan Wagner, the study involves collecting air samples and surface swabs from the internal components of the “Glavbox” apparatus to assess contamination levels. These samples are incubated in nutrient medium within a thermostatic environment for several days to monitor microbial growth. Following the recent “Photobioreactor” experiment, the Aseptic experiment is pivotal in ensuring the reliability of sterile conditions necessary for conducting biotechnological research in microgravity. This research underlines the importance of maintaining contamination-free settings, critical for the advancement of space biotechnology.